𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Molecular cloning of alcohol dehydrogenase genes of the yeastPichia stipitis and identification of the fermentative ADH

✍ Scribed by Passoth, Volkmar; Schäfer, Bernd; Liebel, Birgit; Weierstall, Thomas; Klinner, Ulrich

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
297 KB
Volume
14
Category
Article
ISSN
0749-503X

No coin nor oath required. For personal study only.

✦ Synopsis

Two Pichia stipitis ADH genes (PsADH1 and PsADH2) were isolated by complementation of a Saccharomyces cerevisiae Adh -mutant. The genes enabled the transformants to grow in the presence of antimycin A on glucose, to use ethanol as sole carbon source and made them sensitive to allylalcohol.

The sequences of the genes showed similarities of 70-77% to sequences of ADH genes of Candida albicans, Kluyveromyces lactis, K. marxianus, and S. cerevisiae and about 60% homology to those of Schizosaccharomyces pombe and Aspergillus flavus.

Southern hybridization experiments suggested that P. stipitis has only these two ADH genes. Both genes are located on the largest chromosome of P. stipitis.

PsADH2 encodes for the ADH activity that is responsible for ethanol formation at oxygen limitation. The gene is regulated at the transcriptional level. Moreover, also in cells grown on ethanol, only PsADH2 transcript was found. PsADH1 transcript was detected under aerobic conditions on fermentable carbon sources.

📜 SIMILAR VOLUMES

Molecular evolution ofHox gene regulatio
Molecular evolution ofHox gene regulation: Cloning and transgenic analysis of the lampreyHoxQ8 gene
✍ Carr, Janet L.; Shashikant, Cooduvalli S.; Bailey, Wendy J.; Ruddle, Frank H. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 358 KB 👁 2 views

The mammalian Hox clusters arose by duplication of a primordial cluster. The duplication of Hox clusters created redundancy within cognate groups, allowing for change in function over time. The lamprey, Petromyzon marinus, occupies an intermediate position within the chordates, both in terms of morp

Cloning and identification of HEM14, the
Cloning and identification of HEM14, the yeast gene for mitochondrial protoporphyrinogen oxidase
✍ D. Moira Glerum; Andrey Shtanko; Alexander Tzagoloff; Nadia Gorman; Peter R. Sin 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 373 KB 👁 2 views

A respiratory-defective mutant (C54) of Saccharomyces cerevisiae was found to have a phenotype consistent with a mutation in either mitochondrial protoporphyrinogen oxidase or ferrochelatase. The mutant is grossly deficient in hemes, accumulates protoporphyrin and is rescued by exogenous heme. The i

PermeabilizedArabidopsis protoplasts pro
PermeabilizedArabidopsis protoplasts provide new insight into the chromatin structure of plant alcohol dehydrogenase genes
✍ Paul, Anna-Lisa ;Ferl, Robert J. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 235 KB

New data from permeabilized protoplasts have expanded our view of the 5Ј DNase I hypersensitive area of the Arabidopsis Adh gene derived from nuclei. DNase I hypersensitivity analyses conducted with permeabilized protoplasts from Arabidopsis cell cultures indicates that there are four distinct sites

Molecular diagnosis of McArdle disease:
Molecular diagnosis of McArdle disease: Revised genomic structure of the myophosphorylase gene and identification of a novel mutation
✍ Christian Kubisch; Eva M. Wicklein; Thomas J. Jentsch 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 213 KB 👁 1 views

McArdle disease is a rare autosomal recessive disorder of the muscle glycogen metabolism caused by mutations in the muscle glycogen phosphorylase gene. Until now, a total number of 11 different mutations in the coding region or splice sites of the myophosphorylase gene have been identified. In contr