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Characterization of the Equilibrium Intermediates in Acid Denaturation of Human Stefin B

✍ Scribed by Eva Žerovnik; Roman Jerala; Luise Kroon-Žitko; Vito Turk; Karl Lohner

Book ID
115133801
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
947 KB
Volume
245
Category
Article
ISSN
1432-1327

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✍ Eva Žerovnik; Roman Jerala; Richard Virden; Louise Kroon Žitko; Vito Turk; Jonat 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB 👁 2 views

It has been shown that human stefin B exhibits molten globule intermediates when denatured by acid or GuHCl. In the presence of TFE, it transforms into a highly helical state. In our first study on its folding mechanism (Z ˇerovnik et al., Proteins 32:296-303), the kinetics measured by circular dich