Biphasic denaturation of human placental
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Hui-Chih Hung; Gu-Gang Chang
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Article
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1998
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John Wiley and Sons
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English
โ 289 KB
๐ 1 views
Human placental alkaline phosphatase is a membrane-anchored dimeric protein. Unfolding of the enzyme by guanidinium chloride (GdmCl) caused a decrease of the fluorescence intensity and a large red-shifting of the protein fluorescence maximum wavelength from 332 to 346 nm. The fluorescence changes we