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Multiple Unfolding Intermediates of Human Placental Alkaline Phosphatase in Equilibrium Urea Denaturation

โœ Scribed by Hung, Hui-Chih; Chang, Gu-Gang


Book ID
118514295
Publisher
Biophysical Society
Year
2001
Tongue
English
Weight
399 KB
Volume
81
Category
Article
ISSN
0006-3495

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Biphasic denaturation of human placental
โœ Hui-Chih Hung; Gu-Gang Chang ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 289 KB ๐Ÿ‘ 1 views

Human placental alkaline phosphatase is a membrane-anchored dimeric protein. Unfolding of the enzyme by guanidinium chloride (GdmCl) caused a decrease of the fluorescence intensity and a large red-shifting of the protein fluorescence maximum wavelength from 332 to 346 nm. The fluorescence changes we