𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry

✍ Scribed by Cindy Govaerts; Jennifer Orwa; Ann Van Schepdael; Eugène Roets; Jos Hoogmartens

Book ID
105360178
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
196 KB
Volume
8
Category
Article
ISSN
1075-2617

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A selective reversed phase liquid chromatography/mass spectrometry (LC/MS^n^) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these polypeptide antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on‐line LC/MS^n^ capability. The main advantage of this hyphenated LC/MS^n^ technique is the characterization of novel related substances without time‐consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Identification of lignans by liquid chro
Identification of lignans by liquid chromatography-electrospray ionization ion-trap mass spectrometry
✍ Patrik C. Eklund; M. Josefin Backman; Leif Å. Kronberg; Annika I. Smeds; Rainer 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 314 KB

## Abstract The fragmentation pattern of 30 compounds belonging to different classes of the lignan family was studied by liquid chromatography‐electrospray ionization ion‐trap mass spectrometry. On the basis of the observed fragmentation patterns, identification of different types of lignans was ac

Characterization of endogenous DNA adduc
Characterization of endogenous DNA adducts by liquid chromatography/electrospray ionization tandem mass spectrometry
✍ Ajai K. Chaudhary; Munetaka Nokubo; Thomas D. Oglesby; Lawrence J. Marnett; Ian 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 678 KB

## Abstract Endogenous carcinogens arising from normal metabolism are known to cause modifications to DNA by reacting with bases to form adducts. Malondialdehyde is a well characterized endogenous carcinogen that forms an adduct with 2′‐deoxyguanosine. This adduct has been detected in rat and human

Characterization of explosives by liquid
Characterization of explosives by liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry using electrospray ionization and parent-ion scanning techniques
✍ Bruno Casetta; Fabio Garofolo 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 464 KB

Analytical techniques for the detection of small amounts of explosives (in the picogram range) are now involved in various application. Some of them concern soil, water and air monitoring in order to face environmental problems related to improper handling procedures either in stocking or in wasting

Analysis of Phosphorylated Terpenes by E
Analysis of Phosphorylated Terpenes by Electrospray Ionization Liquid Chromatography–Tandem Mass Spectrometry
✍ Iris Ney; Elke Jäger; Markus Herderich; Peter Schreier; Wilfried Schwab 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 362 KB 👁 2 views

Linalool, nerol, geraniol, citronellol and a-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MSMS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of

Characterization of Oligonucleotide Meta
Characterization of Oligonucleotide MetabolismIn Vivovia Liquid Chromatography/Electrospray Tandem Mass Spectrometry with a Quadrupole Ion Trap Mass Spectrometer
✍ Griffey, Richard H.; Greig, Michael J.; Gaus, Hans J.; Liu, Kenneth; Monteith, D 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 462 KB 👁 2 views

The pattern of nuclease degradation observed for an antisense phosphorothioate oligonucleotide in pig kidney was determined using liquid chromatography/electrospray mass spectrometry (LC/ESI-MS) and LC/ESI-MS/MS with a quadrupole ion trap mass spectrometer. Metabolites were separated by length using