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Characterization of phenolic antioxidants from Maté (Ilex Paraguayensis) by liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry

✍ Scribed by M. Carini; R. Maffei Facino; G. Aldini; M. Calloni; L. Colombo

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
118 KB
Volume
12
Category
Article
ISSN
0951-4198

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✦ Synopsis

Liquid chromatography/ion trap mass spectrometry with an atmospheric pressure chemical ionization (APCI) interface in the negative-ion mode, using collision-induced dissociation (CID) of precursor ions in parallel with UV-diode-array detection (DAD), was applied to the characterization in crude extracts of the water-soluble antioxidant phenolics from Mate ´leaves (Ilex paraguayensis). APCI gives unequivocal information about both the molecular mass of these compounds and some indications about their structure, which were confirmed by the UV-DAD fingerprints and by the diagnostic CID patterns. This allowed the identification of 10 constituents, including the 3 naturally occurring isomers of caffeoylquinic acid (CGA), namely neo-chlorogenic acid, chlorogenic acid and crypto-chlorogenic acid, as well as 3 isomeric dicaffeoyl quinic acids, rutin (quercetin-3-rutinoside), a diglycosyl derivative of luteolin, and 2 isomeric caffeoylglucosides. The aqueous extract of Mate ´, quantitatively analyzed for polyphenol and CGA content (HPLC), was tested for antioxidant activity in both a cell-free system (quenching of the stable free radical DPPH, 2,2diphenyl-1-picryl-hydrazyl) and in a model membrane system (phosphatidylcholine liposomes). The results demonstrate that the extract is a strong radical scavenger, considerably more potent than the prototype chlorogenic acid, and this indicates a cooperative antioxidant interaction among its polyphenol components.

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