An octasaccharide was released from sycamore cell wall rhamnogalacturonan-II (RG-II) by selective acid hydrolysis of the glycosidic linkages of apiosyl residues and purified to homogeneity by gel-permeation and high-performance anion-exchange chromatographies. The octasaccharide l contains a termina
Characterization of pectic polysaccharides from pulse husks
β Scribed by Gudipati Mualikrishna; Rudrapatnam N. Tharanathan
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 219 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0308-8146
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Previous methods of extracting pectin from sugar-beet have used pulp as the starting material. As the temperature and pressure of the pulping process may modify the architecture of the cell wall, we have adapted a relatively non-disruptive method to characterise cell wall material (CWM) isolated dir
Cell-wall material was prepared from unripe tomato fruit, and a pectic polysaccharide extracted with cyclohexanediaminetetraacetic acid. The structure of the purified pectic polysaccharide was examined by sugar and methylation analysis, and was typical of a rhamnogalacturonan from the primary cell w
## Summary Polygalacturonate 4βΞ±βgalacturonosyltransferase (PGAβGalAT), the glycosyltransferase that synthesizes the plant cell wall pectic polysaccharide homogalacturonan, has previously been identified and partially characterized in tobacco membranes. Membrane bound PGAβGalAT catalyzes the transf