The pectic polysacchatide rhamnogalacturonan II (RG-II), which accounts for ~ 20% of the ethanol-precipitable polysacchatides in red wine, has been isolated from wine polysaccharides by anion-exchange chromatography. Four fractions enriched with RG-II were obtained and the RG-II then purified to hom
Structural characterization of the pectic polysaccharide, rhamnogalacturonan-II
β Scribed by Andrew J. Whitcombe; Malcolm A. O'Neill; Wolfram Steffan; Peter Albersheim; Alan G. Darvill
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 817 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0008-6215
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β¦ Synopsis
An octasaccharide was released from sycamore cell wall rhamnogalacturonan-II (RG-II) by selective acid hydrolysis of the glycosidic linkages of apiosyl residues and purified to homogeneity by gel-permeation and high-performance anion-exchange chromatographies. The octasaccharide l contains a terminal nonreducing /3-e-arabinofuranosyl residue linked to position 2 of the a-L-rhamnopyranosyl residue of the aceric acid-containing heptasaccharide 2 that had been previously isolated from RG-II [M.W. Spellman et al. Carbohydr. Res., 122 (1983) 131-153].
π SIMILAR VOLUMES
Rhamnogalacturonan I (RG-I), a pectic polysaccharide isolated from the walls of suspension-cultured sycamore cells, was shown by glycosyl-residue composition analysis to contain D-glucosyluronic acid (GlcpA) residues (1 mol%) and 4-O-methyl-D-glucosyluronic acid (4-O-Me-GlcpA) residues (0.5 mol%). T