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Characterization of P1,P4-Diadenosine 5′-Tetraphosphate Binding on Bovine Aortic Endothelial Cells

✍ Scribed by Shannelle A. Campbell; Aaron J. Kemerling; Richard H. Hilderman

Book ID
115567550
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
85 KB
Volume
364
Category
Article
ISSN
0003-9861

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✍ Jeffrey C. Baker; Myron K. Jacobson 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 846 KB

A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selecti