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Binding and internalization of p1,p4-diadenosine 5'-tetraphosphate by bovine aortic endothelial cells

โœ Scribed by Richard H. Hilderman; Amy T. Fairbank


Book ID
117457245
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
73 KB
Volume
81
Category
Article
ISSN
0300-9084

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A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selecti