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Characterization of the interaction of P1,P4-diadenosine 5′-tetraphosphate with luciferase

✍ Scribed by Luminita H Pojoga; Jana E Moose; Richard H Hilderman


Book ID
116287988
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
1000 KB
Volume
315
Category
Article
ISSN
0006-291X

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Determination of diadenosine 5′,5‴,-P1,P
✍ Jeffrey C. Baker; Myron K. Jacobson 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 846 KB

A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selecti