Characterization of Ovine Hypothalamic Hypophysiotropic TSH-releasing Factor
β Scribed by BURGUS, ROGER; DUNN, THOMAS F.; DESIDERIO, DOMINIC; WARD, DARRELL N.; VALE, WYLIE; GUILLEMIN, ROGER
- Book ID
- 109672623
- Publisher
- Nature Publishing Group
- Year
- 1970
- Tongue
- English
- Weight
- 533 KB
- Volume
- 226
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/226321a0
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π SIMILAR VOLUMES
Amunine, or corticotropin releasing factor (CRF), a 41-peptide amide, has been isolated from ovine hypothalami. Tissues were extracted, defatted, and partitioned prior to being sized by gel permeation. The active zone followed by a sensitive in vitro assay (CRF-mediated corticotropin release from pi
## Abstract This paper illustrates the role that mass spectrometry played in the first elucidation of the primary structure of a hypothalamic releasing factor. It has been demonstrated that ovine TRF has the structure 2βpyrrolidoneβ5βcarboxylylβhistidylβproline amide.