High-performance liquid chromatographic purification of peptide hormones: Ovine hypothalamic amunine (corticotropin releasing factor)
✍ Scribed by J. Rivier; C. Rivier; J. Spiess; W. Vale
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 590 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Amunine, or corticotropin releasing factor (CRF), a 41-peptide amide, has been isolated from ovine hypothalami. Tissues were extracted, defatted, and partitioned prior to being sized by gel permeation. The active zone followed by a sensitive in vitro assay (CRF-mediated corticotropin release from pituitary cells in monolayer cultures quantitated by a specific corticotropin radioimmunoassay) could be purified by reverse-phase high-performance liquid chromatography. Column characteristics (i.e., properties of the derivatized silicas including pore size), selection of mobile phases, and temperature effects are discussed.
' This paper was presented at the International Symposium on HPLC of Proteins and Peptides,