Characterization of Kafirin and Zein Oligomers by Preparative Sodium Dodecyl Sulfate−Polyacrylamide Gel Electrophoresis
✍ Scribed by Nunes, Alexandra; Correia, Isabel; Barros, António; Delgadillo, Ivonne
- Book ID
- 126246453
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 327 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0021-8561
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Various conditions were analyzed and optimized for the preparative elution of proteins from nitrocellulose membranes after transfer from sodium dodecyl sulfate (SDS)-polyacrylamide gels. The efficiency of elution was best using pyridine or acetonitrile elution solvents, intermediate for buffer conta
Capillary sodium dodecyl sulfate-gel electrophoresis, a one-dimensional version of the well-established planar analytical method of SDS-polyacrylamide gel electrophoresis, has proven a powerful new microanalytical method for the separation of protein molecules according to their size. In this paper