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Characterization of homogeneously staining regions in a small cell lung cancer cell line, using in situ hybridization with an MYCN probe

✍ Scribed by Erin Dietzsch; Robyn E. Lukeis; Vickie Vrazas; Suzanne Hasthorpe; Dr. O. Margaret Garson

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 338 KB
Volume: 10
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870100312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The cell line CIPL38 was derived from the pleural effusion of a patient with small cell lung cancer. The karyotype was hyperdiploid and complex with a variable number of marker chromosomes. Two of the markers had large homogeneously staining regions (hsr), which were shown to consist of amplified MYCN by in situ hybridization. One hsr bearing a marker chromosome could not be identified with G‐banding, but the other was situated on a der(14). This was elucidated further with FISH analysis, which enabled the identification of sequences of chromosome I involved in a complex rearrangement with chromosome 14 and the hsr. Genes Chromosom Cancer 10:213–216 (1994). © 1994 Wiley‐Liss, Inc.

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