Detection of 11q13 amplification as the origin of a homogeneously staining region in small cell lung cancer by chromosome microdissection
✍ Scribed by Jia Xu; Tina Tyan; Edward Cedrone; Niramol Savaraj; Nancy Wang
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 640 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Chromosomal homogeneous staining region (hsr), which is a cytogenetic indication of gene amplification, was found in the pleural effusion (BHII) of a patient with small cell lung cancer (SCLC) after failure of multiple drug treatments. Amplification of band I I q I 3 was identified as the origin of the hsr by means of chromosomal microdissection combined with G-banding, DNA amplification by polymerase chain reaction, and fluorescence in situ hybridization (micro-FISH). In situ hybridization with the biotin-labeled DNA probe generated from the hsrs of BHll t o the cell line BHI, which was established from the lymph node metastasis prior t o chemotherapy. revealed the preexistence of I I q I 3 amplification. This ruled out the possibility of therapeutic induction of the I I q I 3 amplification. However, the hsr-bearing marker chromosomes were identified by the micro-FISH approach as der(21)(Xqter+Xq24hsr(I l)(q13)::21pl I+2lqter) in BHll but as der(l l)t(3;1 l)(q21;q13)hsr(l l)(q13) in BHI. This suggests that I I q I 3 DNA sequence amplification may occur first and is then followed by various types of structural rearrangements. FISH analysis with INWFGF3 and HSTIIFGF4 probes revealed that these protooncogenes were coamplified in the hsrs of BHI and BHII. The results obtained suggest that I I q I 3 amplification and the successive structural rearrangement may play an important role in the progression of the disease and its therapeutic response.