✦ LIBER ✦
Use of fluorescent in situ hybridization to confirm trisomy of chromosome region 1q32-qter as the sole karyotypic defect in a colon cancer cell line
✍ Scribed by Darren K. Griffin; Sarah E. A. Leigh; Dr. Joy D. A. Delhanty
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 278 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
The sole chromosome defect in a colon cancer cell line derived from a patient with inherited nonpolyposis colorectal cancer was karyotypically designated as 46,XY,-I3,+der( I3)t( I; I3)(q32. I ;p I I) on the basis of banding homology. We have obtained molecular confirmation that the additional chromosome material is derived from chromosome region I q32-qter by the use of a highly specific fluorescent in situ hybridisation technique on G-banded chromosomes and also by Southern hybridisation.