Characterization of a Human NK1 Tachykinin Receptor in the Astrocytoma Cell Line U 373 MG
✍ Scribed by E. Heuillet; J. Ménager; V. Fardin; O. Flamand; M. Bock; C. Garret; A. Crespo; A. M. Fallourd; A. Doble
- Book ID
- 111177766
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 982 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-3042
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The neurokinin-1 (NK-1, substance P) receptor belongs to the class of seven transmembrane domain (7-TM) receptors that interact with cellular effector systems via guanine nucleotide binding regulatory proteins (G-proteins). In this study, coupling mechanisms of functional NK-1 receptoirs endogenousl
Variations in intracellular free calcium concentration (A1Cazfli) were measured in intact and isolated human astrocytoma cells (U373 MG) loaded with fura-2 acetoxymethylester. Microperfusion of 50 nM substance P (SP), applied for 1 s, increased [Ca" ' Ii by 351 nM from a stable basal level of [Ca2+l