Functional characterization of neurokinin-1 receptors on human U373MG astrocytoma cells

✍ Scribed by Harald R. Eistetter; Ann Mills; Rachel Brewster; Sami Alouani; Christine Rambosson; Eric Kawashima

• Book ID: 102845808
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 859 KB
• Volume: 6
• Category: Article
• ISSN: 0894-1491
• DOI: 10.1002/glia.440060203

✦ Synopsis

The neurokinin-1 (NK-1, substance P) receptor belongs to the class of seven transmembrane domain (7-TM) receptors that interact with cellular effector systems via guanine nucleotide binding regulatory proteins (G-proteins). In this study, coupling mechanisms of functional NK-1 receptoirs endogenously expressed in a human astrocytoma cell line (U373MG) were analyzed. Stimulation with substance P (SP) resulted in 1) a rapid increase in inositol 1,4,5-trisphosphate (IP,) synthesis; 2) a rise in cytosolic free calcium concentration (ICa2'l,); 3) iiiduction of immediate early gene transcription as monitored by c-fos and c j u n expression; and 4) a significant increase in de novo DNA synthesis. Thus, the functional responses induced by stimulation of NK-1 receptors on U373MG strongly correlate with those observed after treatment of primary astrocytes with SP and make U373MG cells a useful in vitro model system for the analysis of NK-1 receptor function on astrocytes ii? vivo. o 1992 Wiley-Liss, Inc.