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Substance P-Evoked Calcium Mobilization and Ionic Current Activation in the Human Astrocytoma Cell Line U 373 MG: Pharmacological Characterization

✍ Scribed by L. Pradier; E. Heuillet; J. P. Hubert; M. Laville; S. Le Guern; A. Doble

Book ID: 111178033
Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 989 KB
Volume: 61
Category: Article
ISSN: 0022-3042
DOI: 10.1111/j.1471-4159.1993.tb09826.x

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✍ A. Bordey; P. Feltz; J. Trouslard 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 629 KB

Variations in intracellular free calcium concentration (A1Cazfli) were measured in intact and isolated human astrocytoma cells (U373 MG) loaded with fura-2 acetoxymethylester. Microperfusion of 50 nM substance P (SP), applied for 1 s, increased [Ca" ' Ii by 351 nM from a stable basal level of [Ca2+l