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Characterization of a BALB/c 3T3 preadipose cell mutant with altered Na+K+Cl− contransport activity

✍ Scribed by Ilene Sussman; Thomas G. O'Brien

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
730 KB
Volume
124
Category
Article
ISSN
0021-9541

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✦ Synopsis

A BALB/c 3T3 cell mutant (3T3-E12) was isolated by its ability to survive at a low extracellular K+ concentration (0.14 mM). The growth rate of mutant cells was less dependent on external K+ than parental cells. Analysis of potassium transport revealed that 3T3-E12 cells have a decreased activity of the furosemide-sensitive Na+K+Cl- cotransport system, both in the efflux and influx modes. This is shown to be a result of a decrease in the apparent affinity of the transport system for K+ and Na+, but not Cl-. Upon exposure to the phorbol ester 12-0-tetradecanoyl-phorbol-13-acetate (TPA), BALB/c 3T3 cells exhibited a maximal volume decrease of 20%, while mutant cells shrunk by only 7%, suggesting that regulation of cell volume, at least four exposure to a tumor promoter, is impaired in mutant cells compared to parental 3T3 cells.

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