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Characteristics of Migration Patterns of DNA Oligomers in Gels and the Relationship to the Question of Intrinsic DNA Bending

โœ Scribed by Haran, Tali E.; Cohen, Ilana; Spasic, Alexander; Yang, Kevin; Mohanty, Udayan

Book ID
126249201
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
180 KB
Volume
126
Category
Article
ISSN
0002-7863

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Curved D N A fragments have a reduced electrophoretic mobility in polyacrylamide gels. The retardation in gels is extremely sensitive to small structural variations which influence the D N A helix axis. This gel assay can also be used to detect very small structural variations in DNA sequences which