The migration anomaly of DNA fragments in polyacrylamide gels allows the detection of small sequence-specific DNA structure variations
✍ Scribed by Dr. Stephan Diekmann
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 713 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Curved D N A fragments have a reduced electrophoretic mobility in polyacrylamide gels. The retardation in gels is extremely sensitive to small structural variations which influence the D N A helix axis. This gel assay can also be used to detect very small structural variations in DNA sequences which are not curved: The noncurved seguences of interest can be combined with curved stretchesin phase with the helix turn. Using such sequence constructions, even subtle influences on the DNA helix axis can be detected. Experiments of this kind allow the determination of a relative order of sequence-specific DNA twist and wedge angles.