Characterisation of mannoproteins from yeast and mycelial forms of Mucor rouxii

✍ Scribed by Haruki Yamada; Masao Hiraiwa; Toshio Miyazaki

• Book ID: 102991023
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 783 KB
• Volume: 119
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(83)84052-x

✦ Synopsis

Mannoprotein was isolated from yeast (Y) and mycelial (M) cells of Mucor rouxii by affinity chromatography on concanavalin A Sepharose. Mannoprotein-Y contained only mannose, whereas mannoprotein-M contained mannose and a small proportion of fucose. Each mannoprotein contained -50% of protein. The main carbohydrate products of p-elimination reactions of the mannoproteins were cz-( 1+2)-linked mannobiose (24% from Y, 15% from M) and polysaccharide material (57% from Y, 79% from M). Polysaccharide-Y consisted of a-(l-+2)-linked, 3,6-di-O-substituted, and non-reducing terminal mannopyranosyl residues, whereas polysaccharide-M contained, in addition, fucopyranosyl non-reducing end-groups and a-(l-+6)-linked mannopyranosyl residues. Acetolysis of polysaccharide-Y gave mainly mannotetraose, mannotriose, and mannobiose, whereas polysaccharide-M gave mainly mannose.