𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Changes in the sulfation extent of membrane-associated proteoglycans produced by sertoli cells in culture

✍ Scribed by Juan Pablo Rodríguez

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
624 KB
Volume
57
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Sertoli cells in culture synthesize two different membrane-associated proteoglycans (MA-PG): a proteoglycan containing heparan sulfate (HS) and chondroitin sulfate (CS) glycosaminoglycan (GAG) chains and a CS-PG containing only CS-GAG chains. The structure of these molecules is regulated by the presence of fetal calf serum (FCS) in the culture medium. Changes in the concentration of FCS resulted in changes in the total 35S04 incorporation into MA-PG and a shift in the elution profile of each component subjected to ion-exchange chromatography. Thus, without FCS, the incorporation was low, while in 1% and 10% FCS, the uptake of the precursor was 1.7 and 4.5 times higher, respectively. MA-PG synthesized by Sertoli cells cultured in 10% FCS eluted from DEAE-Sephacel columns at higher salt concentration than the MA-PG synthesized by cells cultured in 0% or 1% FCS. Double-labeled experiments showed that the 35S04/3H-glucosamine ratio incorporated into MA-PG produced by Sertoli cells, increased from 17.6 to 23.6 and 50.9 in cells cultured at 0, 1, and 10% FCS, respectively. However, the presence of FCS affected neither the hydrodynamic size nor the chemical nature of GAG chains of MA-PG. These results show that changes in the FCS concentration promote changes in the sulfation extent of MA-PG molecules produced by Sertoli cells.

📜 SIMILAR VOLUMES

Collagen increases the synthesis of memb
Collagen increases the synthesis of membrane-associated proteoglycans produced by sertoli cells
✍ J. Pablo Rodriguez; Jose J. Minguell 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 471 KB

Sertoli cells in culture produce two isoforms of proteoglycans which are found in the culture medium and associated with the cell membrane. The amount of both types of proteoglycans increased when Sertoli cells were plated on type f collagen-coated dishes as compared to uncoated dishes. The effect i

Modifications in the synthesis of membra
Modifications in the synthesis of membrane-associated chondroitin sulfate proteoglycans in hemopoietic progenitor cells are accompanied by alterations in their adhesive properties
✍ Paulette Conget; José J. Minguell 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 992 KB

## Abstract In vitro studies in our laboratory have indicated that murine hemopoietic progenitor cell (HPC) lines, irrespective of their differentiation stage, synthesize and accumulate in the cell membrane a unique species of chondroitin sulfate proteoglycan (CS‐PG). It has been postulated that CS

In vitro conditions affecting the synthe
In vitro conditions affecting the synthesis of sulfated proteoglycans by normal and rheumatoid synovial cells in culture
✍ Joanne M. Marsh; David W. Roback; Garry T. Ross 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 762 KB

## Abstract In vitro conditions affecting synthesis of sulfated proteoglycans by cell suspensions derived from monolayer cell cultures of normal and rheumatoid synovial tissue were examined. The capacity of cells to synthesize proteoglycans was estimated by the incorporation of ^35^S–sulfate into c