In vitro conditions affecting the synthesis of sulfated proteoglycans by normal and rheumatoid synovial cells in culture
✍ Scribed by Joanne M. Marsh; David W. Roback; Garry T. Ross
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 762 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Abstract
In vitro conditions affecting synthesis of sulfated proteoglycans by cell suspensions derived from monolayer cell cultures of normal and rheumatoid synovial tissue were examined. The capacity of cells to synthesize proteoglycans was estimated by the incorporation of ^35^S–sulfate into cetylpyridinium chloride–precipitable material. Synthesis of sulfated proteoglycans was maximal during log phase, and after 2–3 hours of recovery from disaggregation. Normal synovial cells appeared to be more sensitive to changes in serum concentration than were rheumatoid synovial cells, but rheumatoid synovial cells were more sensitive to changes in cell density. The proportion of newly synthesized extracellular proteoglycans increased with the duration of incubation in ^35^S–sulfate.