Changes in the buffer-soluble proteins during maturation of the wheat kernel

✍ Scribed by D. W. Rainey; Donald C. Abbott

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 411 KB
• Volume: 22
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.2740221202

✦ Synopsis

Head samples of two cultivars of hard red winter wheat were taker2 f.om 4 days after heading until mature. Buffer extracts (0.025 M-phosphate, 0 . 5 M-NaCI, pH 6.8) of samples from the various stages of development were analysed for protein content and submitted to immunoelectrophoretic analysis (i.e.a.). Results were similar for both cultivars. Protein content of extracts of the more immature samples was higher than for the more mature samples (dry weight basis), but proteins corresponding to those of mature wheat were lower in the immature samples. I.e.a., using complete anti-mature-wheat serum and the same serum absorbed by extracts from different maturity stages, showed the development of 7 new proiein componenis during the course of development of the wheat kernel. Results also suggested the presence, in immature wheat, of proteins antigenically different from those in mature wheat.