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Protein changes during maturation of the free-living nematode,Panagrellus silusiae

✍ Scribed by Chow, H. Hu ;Pasternak, J.

Publisher: John Wiley and Sons
Year: 1969
Tongue: English
Weight: 495 KB
Volume: 170
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401700107

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✦ Synopsis

The free-living nematode Panagrellus sibsiae can be g o w n synchronously through the postpartum period of its life cycle. This period is divided into four phases which in sequence are called the L2, L3, L4 and adult stages. Homogenates from each stage were analyzed electrophoretically in order to discover whether protein changes occur during postembryonic development. Protein patterns in acrylamide gels from L2, L3, L4 and adult stages show no differences in the number of bands (16), although there are differences in the intensity of certain bands at various stages.

Definite stage specific patterns were observed when enzyme activity was assayed histochemically. L4 larvae show five sites of lactate dehydrogenase activity in polyacrylamide gel; three of these are found in each of the other stages. L3 larval extracts have five sites of malate dehydrogenase activity; L2 larvae and adults share one of

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