A simple, sensitive, and quantitative method for the analysis of nonhistone chromosomal proteins separated by two-dimensional polyacrylamide gel electrophoresis was established. Chromosomal proteins were first fractionated by isoelectrofocusing gel electrophoresis. By this method the histones were f
Challenges Related to Analysis of Protein Spot Volumes from Two-Dimensional Gel Electrophoresis As Revealed by Replicate Gels
โ Scribed by Grove, Harald; Hollung, Kristin; Uhlen, Anne Kjersti; Martens, Harald
- Book ID
- 127344871
- Publisher
- American Chemical Society
- Year
- 2006
- Tongue
- English
- Weight
- 939 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1535-3893
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The products of the ras genc family are related proteins at a molecular weight of 2 I kDa, designated p2 I . In the present study we used two-dimcn~ional gcl electrophoresis to compare p2 1 proteins from fivc different normal and malignnnt cell lines. Using a known protein (%labeled translation init