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Fluorescamine staining of nonhistone chromatin proteins as revealed by two-dimensional polyacrylamide gel electrophoresis

✍ Scribed by G. Jackowski; C.C. Liew

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
1017 KB
Volume
102
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, sensitive, and quantitative method for the analysis of nonhistone chromosomal proteins separated by two-dimensional polyacrylamide gel electrophoresis was established. Chromosomal proteins were first fractionated by isoelectrofocusing gel electrophoresis. By this method the histones were focused off the isoelectric focusing gel (pl > 9.0). The nonhistone chromatin proteins were further separated in the second dimension by sodium dodecyl sulfate-polyacrylamide gel electrophoresis according to their molecular weights. Fluorescamine staining of nonhistone chromatin proteins was carried out following twodimensional polyacrylamide gel electrophoresis. This staining did not interfere with the isoelectric points of the proteins. The quantitation of proteins based on fluorescamine staining versus Coomassie brilliant blue staining was also discussed.

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