Analysis of fibroblast proteins from patients with Duchenne muscular dystrophy by two-dimensional gel electrophoresis
β Scribed by Rosenmann, E.; Kreis, C.; Thompson, R. G.; Dobbs, M.; Hamerton, J. L.; Wrogemann, K.
- Book ID
- 109727184
- Publisher
- Nature Publishing Group
- Year
- 1982
- Tongue
- English
- Weight
- 651 KB
- Volume
- 298
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/298563a0
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The products of the ras genc family are related proteins at a molecular weight of 2 I kDa, designated p2 I . In the present study we used two-dimcn~ional gcl electrophoresis to compare p2 1 proteins from fivc different normal and malignnnt cell lines. Using a known protein (%labeled translation init
Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the