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Cesium chloride density distributions in preparative equilibrium ultracentrifugation

✍ Scribed by Peter Rüst

Publisher
Wiley (John Wiley & Sons)
Year
1968
Tongue
English
Weight
802 KB
Volume
6
Category
Article
ISSN
0006-3525

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✦ Synopsis

A computer program has been developed for calculating equilibrium centrifugation density distributions by direct integration of tabulated data, which eliminates curve fitting. Error calculations showed that a high accuracy is required for the measurement of the data needed to determine a density distribution either by computation or by direct measurements. In particular, the variation in drop size during the fractionation of the contents of a centrifuge tube have been investigated by computer simulation. A direct measurement of the drop size indicated that it may increase somewhat during collection. In addition, plots are presented of the dependence upon seven parameters of the centrifugation required to approach equilibrium to a desired degree (baaed on the formula of Van Holde and Baldwin).

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