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Effects of pressure on the composition density distribution in the ultracentrifuge

✍ Scribed by Dan S. Sharp; James B. Ifft

Publisher
Wiley (John Wiley & Sons)
Year
1982
Tongue
English
Weight
459 KB
Volume
21
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The effects of pressure on the composition density distribution in a binary density gradient at sedimentation equilibrium in the analytical ultracentrifuge are rigorously examined. A computer algorithm is described for the necessary iterative computations. The pressure effect is found to be significant in runs where a long column length, high angular velocity, and a salt with a high pressure correction coefficient are simultaneously employed. Such conditions are sometimes encountered in current studies in which high precision is required to measure the compressibility of proteins.

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