Ceramic-supported Hybridomas for Continuous Production of Monoclonal Antibodies
โ Scribed by A. MARCIPAR; P. HENNO; E. LENTWOJT; A. ROSETO; G. BROUN
- Book ID
- 118721154
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 193 KB
- Volume
- 413
- Category
- Article
- ISSN
- 0890-6564
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๐ SIMILAR VOLUMES
Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical dass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 X 1 O7 cells/ml was achie
A fixed bed perfusion system for hybridoma cell immobilization is presented. The system consists of a culturing vessel (300 ml total volume) in which polyurethane (PU) sponges in the form of small cubes of about 5 mm sides are packed. Cells are immobilized by physical entrapment in the foam matrix.