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Cellular localisation of the clamp protein during DNA replication

✍ Scribed by Kritaya Kongsuwan; Brian P Dalrymple; Gene Wijffels; Phillip A Jennings


Book ID
109327693
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
414 KB
Volume
216
Category
Article
ISSN
0378-1097

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Cloning of the replication gene P of bac
✍ Klinkert, JΓΆrg ;Klein, Albrecht πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 844 KB

A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/