CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS

✍ Scribed by L.A. Sadofiev; N.S. Nikolaenko; N.V. Kalmykova; O.I. Podgornaya

• Book ID: 102966827
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 523 KB
• Volume: 25
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1999.0487

✦ Synopsis

Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with M(R)of 250, 170 and 140 kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140 kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin beta1 chain. Ab p140 and commercial monoclonal antibodies against alpha(2)chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140 kDa protein is probably the alpha(2)integrin chain. The alpha(2)chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent.