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Cell permeabilization as a tool for measurement of intracellular enzyme activity in a flow-injection system

✍ Scribed by Gert Blankenstein; Maria-Regina Kula


Book ID
118509095
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
656 KB
Volume
248
Category
Article
ISSN
0003-2670

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Chymotrypsin, covalently immobilized to the surface of an IrO,-coated titanium electrode, responds potentiometrically to various substrates. A flow-injection system is described for assay of the activity of the immobilized enzyme with N-benzoyl-L-tyrosine ethyl ester as substrate and an ultraviolet