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A flow-injection system for assay of the activity of an immobilized enzyme chemically-modified electrode

✍ Scribed by James A. Osborn; Alexander M. Yacynych; David C. Roberts

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
402 KB
Volume
183
Category
Article
ISSN
0003-2670

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✦ Synopsis

Chymotrypsin, covalently immobilized to the surface of an IrO,-coated titanium electrode, responds potentiometrically to various substrates. A flow-injection system is described for assay of the activity of the immobilized enzyme with N-benzoyl-L-tyrosine ethyl ester as substrate and an ultraviolet detector. Least-squares fits of peak height vs. time typically yield correlation coefficients of 0.999 and standard errors of estimate of 0.0043 absorbance for a total absorbance change of about 0.130. Slopes of such plots vary linearly with enzyme activity.

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