Cell-mediated cytotoxicity to chemically-induced rat tumours

✍ Scribed by Margot Zöller; Michael R. Price; Robert W. Baldwin

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: French
• Weight: 867 KB
• Volume: 16
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910160409

✦ Synopsis

Abstract

Cell‐mediated immune reactions against carcinogen‐induced rat tumours were demonstrated by the in vitro microcytotoxicity test. Lymph‐node cells from syngeneic rats immunized against individual aminoazo‐dye‐induced hepatomas or 3‐methylchol‐anthrene‐induced sarcomas were cytotoxic for cells of the immunizing tumour compared with the effects of normal lymph‐node cells. Only a low level of cross‐reactivity was detectable against target cells derived from other tumours. Conversely, lymph‐node cells from tumour‐bearing rats were cross‐reactive against a range of tumour cells, suggesting that the tumour‐bearer was sensitized against a common antigen (s) as well as the individually specific antigen associated with these tumours. Lymph‐node cells were reactive at an early time (from day 2) after tumour implantation and their cytotoxic potential persisted until terminal stages of tumour growth. Spleen cells from tumour‐bearer and tumour‐immune donors also exhibited comparable cytotoxic reactivities of the same specificity as that shown by lymph‐node cell preparations. These findings are discussed in relation to the development of cell‐mediated immune responses in the tumour‐bearing rat and in immunized rats which are capable of rejecting transplanted tumour cells.