Cell-free synthesis of lipoprotein in systems programmed by free and membrane-bound polysomes of Escherichia coli
✍ Scribed by Daniel Baty; Jean-Marie Pages; Yolande Berthois; Claude Lazdunski
- Book ID
- 109311666
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 247 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
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## Abstract Crude cell‐free extracts of Escherichia coli AB 259 Hfr 3000, thi‐perform multistep conversion of ^14^C‐uracil to ^14^C‐UTP in vitro in the presence of D‐ribose/or ribose‐5‐phosphate/, ATP and ATP‐regenerating system. ATP concentration is crucial for significant ^14^C‐UTP production. Qu
Polysomes from Krebs II ascites and 3T3 cells were separated into three populations by using a sequential extraction method. Free polysomes were released by using a combination of low salt (25 mM KCl) and NP-40 detergent in the lysis buffer. The cytoskeletal bound polysomes were subsequently release