Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems

Expression of functional Candida antarct

Expression of functional Candida antarctica lipase B in a cell-free protein synthesis system derived from Escherichia coli

✍ Chang-Gil Park; Tae-Wan Kim; In-Seok Oh; Jae Kwang Song; Dong-Myung Kim 📂 Article 📅 2009 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 161 KB 👁 2 views

## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led