β Scribed by Mari Tabuchi; Mami Hino; Yasuo Shinohara; Yoshinobu Baba
No coin nor oath required. For personal study only.
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Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was u
## Abstract Cellβfree protein synthesis is a useful research tool and now stands poised to compete with in vivo expression for commercial production of proteins. However, both the extract preparation and protein synthesis procedures must be scaled up. A key challenge is producing the required amoun
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