𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cell-free protein synthesis: A step on the path to clinical production

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
46 KB
Volume
108
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Dihydrofolate reductase synthesis in the
Dihydrofolate reductase synthesis in the presence of immobilized methotrexate. An approach to a continuous cell-free protein synthesis system
✍ Ewa Marszal; William H. Scouten πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 577 KB

Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible

Safe two-plasmid production for the firs
Safe two-plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one-step protocol
✍ Xiaobin Lu; Laurent Humeau; Vladimir Slepushkin; Gwendolyn Binder; Qiao Yu; Tati πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 398 KB

## Abstract We report the design of a unique two‐plasmid production system for the first lentiviral vector to be evaluated in humans, VRX496. VRX496 is an optimized VSV‐G pseudotyped vector derived from HIV‐1 that expresses antisense to the HIV envelope gene. We found that a two‐plasmid approach to