𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Energizing cell-free protein synthesis with glucose metabolism

✍ Scribed by Kara A. Calhoun; James R. Swartz


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
335 KB
Volume
90
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Cell-free protein synthesis on a microch
✍ Mari Tabuchi; Mami Hino; Yasuo Shinohara; Yoshinobu Baba πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 124 KB
Rational improvement of cell-free protei
✍ Anders Pedersen; Kristofer Hellberg; Johan Enberg; B. GΓΆran Karlsson πŸ“‚ Article πŸ“… 2011 πŸ› Elsevier 🌐 English βš– 415 KB

Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was u

Cell-free synthesis of proteins that req
✍ Kurtis G. Knapp; Aaron R. Goerke; James R. Swartz πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 223 KB

## Abstract The primary objective of this work was to create a cell‐free protein synthesis extract that produces proteins requiring disulfide bonds while using glucose as an energy source. We attempted to avoid using iodoacetamide (IAM) to stabilize the required oxidizing thiol redox potential, sin

Prolonging cell-free protein synthesis w
✍ Dong-Myung Kim; James R. Swartz πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 474 KB

A new approach for the regeneration of adenosine triphosphate (ATP) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate. This approach was demonstrated in a batch system derived from Escherichia coli. Contrary to the con

Coping with complexity: Machine learning
✍ Filippo Caschera; Mark A. Bedau; Andrew Buchanan; James Cawse; Davide de Lucrezi πŸ“‚ Article πŸ“… 2011 πŸ› John Wiley and Sons 🌐 English βš– 450 KB

## Abstract Biological systems contain complex metabolic pathways with many nonlinearities and synergies that make them difficult to predict from first principles. Protein synthesis is a canonical example of such a pathway. Here we show how cell‐free protein synthesis may be improved through a seri