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Cell-dislodging methods under serum-free conditions

✍ Scribed by H. J. Cruz; E. M. Dias; J. L. Moreira; M. J. T. Carrondo

Book ID
105954111
Publisher
Springer
Year
1997
Tongue
English
Weight
838 KB
Volume
47
Category
Article
ISSN
1432-0614

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## Abstract Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement a