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Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions

✍ Scribed by Tiago G. Fernandes; Ana M. Fernandes-Platzgummer; Cláudia Lobato da Silva; Maria Margarida Diogo; Joaquim M. S. Cabral

Book ID
106364521
Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
420 KB
Volume
32
Category
Article
ISSN
0141-5492

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## Abstract The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA