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Lymphoma cell culture under serum-free conditions

✍ Scribed by Yu-cheng Dai; Xin-hua Wang; Nazir Jamal; Hans A. Messner

Book ID
112803752
Publisher
Society for In Vitro Biology
Year
1993
Tongue
English
Weight
295 KB
Volume
29
Category
Article
ISSN
1475-2689

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## Abstract Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement a