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Cell cycle dependent activities of DNA polymerases .alpha. and .delta. in Chinese hamster ovary cells

โœ Scribed by Marraccino, Robert L.; Wahl, Alan F.; Keng, Peter C.; Lord, Edith M.; Bambara, Robert A.


Book ID
126082278
Publisher
American Chemical Society
Year
1987
Tongue
English
Weight
932 KB
Volume
26
Category
Article
ISSN
0006-2960

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The major pol alpha activity of CHO cells was purified 2 800-fold to near homogeneity and was characterized with respect to its physical and catalytic properties. The purified enzyme, upon analysis in denaturing 'activity' gels, displayed a major, 120 kilodalton, catalytically active core and two mi