Cell cycle dependency of rice α-amylase production in a recombinant yeast

✍ Scribed by Keiji Uchiyama; Masaaki Morimoto; Yasuhiro Yokoyama; Suteaki Shioya

• Book ID: 101242385
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 342 KB
• Volume: 54
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970505)54:3<262::aid-bit7>3.0.co;2-d

✦ Synopsis

The cell cycle dependency of rice ␣-amylase production in Saccharomyces cerevisiae was investigated using synchronous and arrested cultures. The results of two separate synchronous cultures, using ␣-mating factor and a cdc28 mutant, indicated that the rice ␣-amylase-specific production rate is not constant during the cell cycle. The specific production rates during G1, S, and M phases were then ascertained by inhibiting the progression of the cell cycle using ␣-mating factor, hydroxyurea, and nocodazole, respectively. The specific production rate was found to be maximal during the M phase. The increase in the specific production rate during the M phase was confirmed from the accumulation of M-phase cells using a cdc15 mutant. The intracellular content of rice ␣-amylase was also measured during the cell cycle. Like the specific production rate, the intracellular content was found to fluctuate throughout the cell cycle, and to reach a maximum during M phase.