Cell free extracts prepared from Xenopus eggs are one of the most powerful in vitro systems to analyze cell cycle-regulated mechanisms such as DNA replication, nuclear assembly, chromosome condensation, or spindle formation. Xenopus embryos can complete several synchronous cell cycles in the absence
Cell Cycle Analysis and Synchronization of theXenopusCell Line XL2
β Scribed by Rustem Uzbekov; Isabelle Chartrain; Michel Philippe; Yannick Arlot-Bonnemains
- Book ID
- 115602992
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 280 KB
- Volume
- 242
- Category
- Article
- ISSN
- 0014-4827
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## Abstract A new procedure to determine the durations of cell cycle phases is based on the autoradiography of labeled nuclei and a standard chemical determination of DNA content per cell. It has been used to study the cell cycle timing of a human established cell line, EUE cells, so far poorly cha