Immunochemical detection of cell cycle synchronization in a human erythroid cell line, K562

✍ Scribed by Paul E. Wanda

• Book ID: 104630133
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 522 KB
• Volume: 18
• Category: Article
• ISSN: 1573-0603
• DOI: 10.1007/bf00122165

✦ Synopsis

Cells of the human erythroleukemic line K562 can be manipulated by culture conditions to arrest within the GI phase of the cell cycle and subsequently to enter S phase synchronously after release from G1. Cell cultures subjected to serum deprivation and hydroxyurea (HU) treatment demonstrated less than 5% of the cells to be in S phase. Four hours after release from HU, 63% of the cells were in S phase, as detected by immunofluorescence. This protocol offers a method for synchronization of K562 cells at the GI/S border and a technique for detection of S-phase cells without the use of radioisotopes or flow cytometry instrumentation.